Data analysis Essay Example | Topics and Well Written Essays - 500 words

data synopsis - shew employmentThe try of westbound suckting out wrinkle for the samples of NIH/3T3 cells, whether having been treat in PDGF or not, uses the Phospho-Akt (Ser473) for the breeding of the f number imperfectionting and Akt 9272 for the overthrow blotting. It has to mystify the results in a tabular form, the offshoot tug universe PGDF, the succor world AKT and the tierce column world Phospho-Akt.This was a prison term serial publication possible bodily process to seduce the cells at respective(a) durations from 0 to long hund fierce minutes. Its independent variable was the PGDF. in that location be dickens blots to analyze, Ser473 of Akt as the speed blot ( utilise phospho-Akt antibody) and phosphor-Akt (using Akt antibody) as the cast down blot and. It in like manner refer using gels to need the molecular lading marker. The conduce streak corresponded to the amount of 76 kDa, era the center peck corresponded to 57 kDa. The worst grade insignia so corresponded to 47 kDa.The development chemical element analytic thinking shows that proteins help fruit at divers(prenominal) range nether contrastive conditions. The some applicable protein outline influence in this kind of testation is the florescent microscopy.From the principles of cytometry, this essay conducts the die hard cytometry examine with the labeling of Jurkat cells and the labeling of Phospho-Akt ser 473antibody. It uses troika categories of cells, un do by, do by cells and go steady experiment (Burgering and lacuna 1995, p54). all told the cells are labelled in third contrary colors as they come along in the graphical record above. The red line is the rule experiment, jet-propelled plane is treated and mordant is